GenomeSpace Tools and Data Sources
Project Website: http://www.broadinstitute.org/achilles
Datasets from Project Achilles are now linked to GenomeSpace. Project Achilles is creating a genome-wide catalog of tumor dependencies, to identify vulnerabilities associated with genetic and epigenetic alterations. Individual genes in hundreds of genomically characterized cancer cell lines are silenced using a genome-wide, pooled shRNA library. Genes that affect cell survival are identified using advanced computational methods, including ATARiS (Analytic Technique for Assessment of RNAi by Similarity). The publicly available 2011 v2.0 analysis is on 102 cell lines that include 25 ovarian, 18 colon, 13 pancreatic, 9 esophageal, 8 non-small-cell lung cancer (NSCLC), and 6 glioblastoma cancer cell lines. Release of v2.4 analyzing 216 cell lines is scheduled.
To send data, click on the GenomeSpace icon next to the dataset name.
The 2011 PNAS publication on v2.0 data is titled Systematic investigation of genetic vulnerabilities across cancer cell lines reveals lineage-specific dependencies in ovarian cancer. Project Achilles data are complemented by that of the Cancer Cell Line Encyclopedia and The Cancer Genome Atlas, which provide data from cancer cell lines and from primary tumors, respectively. Project Achilles is a collaboration between the Broad Institute of MIT and Harvard and the Dana-Farber Cancer Institute.